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Rabbit Platelet Factor 4 (PF4) ELISA Kit

SKU:
RBDL00061
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P83470
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CXCL4,SCYB4,Chemokine C-X-C-Motif Ligand 4,Oncostatin-A,Iroplact
Reactivity:
Rabbit
Sample Type:
Serum And Platelet-Poor Plasma
€649
Frequently bought together:

Description

system_update_altDatasheet

Rabbit Platelet Factor 4 (PF4) ELISA Kit

The Rabbit Platelet Factor 4 (PF4) ELISA Kit from Assay Genie is specifically designed for the quantitative detection of PF4 levels in rabbit serum, plasma, and cell culture supernatants. This kit offers high sensitivity and precision, ensuring accurate and reliable results for a variety of research applications.Platelet Factor 4 is a key protein involved in blood clotting and inflammation processes. Abnormal levels of PF4 have been associated with various health conditions, such as thrombosis, cardiovascular diseases, and autoimmune disorders.

As such, this ELISA kit serves as a valuable tool for investigating the role of PF4 in disease pathogenesis and potential therapeutic interventions.Trust Assay Genie's Rabbit Platelet Factor 4 (PF4) ELISA Kit for robust and dependable results in your research endeavors. Order yours today and unlock new insights into the biology of PF4 and its implications in health and disease.

Product Name:Rabbit Platelet Factor 4 (PF4) ELISA Kit
Product Code:RBDL00061
Size:96 Assays
Target:PF4
Synonyms:CXCL4, SCYB4, Chemokine C-X-C-Motif Ligand 4, Oncostatin-A, Iroplact
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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