null

Rabbit Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit

SKU:
RBDL00060
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P34007
Sensitivity:
10.8pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
PDGF-A,PDGF1,Platelet Derived Growth Factor Alpha Polypeptide
Reactivity:
Rabbit
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rabbit Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit

The Rabbit Platelet-Derived Growth Factor Subunit A (PDGF-A) ELISA Kit is designed for the precise measurement of PDGF-A levels in rabbit serum, plasma, and cell culture supernatants. This kit is known for its superior sensitivity and specificity, ensuring accurate and consistent results, making it suitable for various research applications.PDGF-A is a key growth factor that plays a crucial role in cell growth, differentiation, and wound healing. It is involved in various biological processes such as tissue repair, angiogenesis, and development, making it a valuable biomarker for studying these processes and potential therapeutic interventions.

With the Rabbit PDGF-A ELISA Kit, researchers can confidently analyze PDGF-A levels in rabbit samples, gaining valuable insights into cellular mechanisms and disease pathways. This kit is an essential tool for advancing research in areas such as regenerative medicine, cancer biology, and vascular biology.

Product Name:Rabbit Platelet Derived Growth Factor Subunit A (PDGFA) ELISA Kit
Product Code:RBDL00060
Size:96 Assays
Target:PDGFA
Synonyms:PDGF-A, PDGF1, Platelet Derived Growth Factor Alpha Polypeptide
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products