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Rabbit Peroxisome Proliferator Activated Receptor Gamma (PPARg) ELISA Kit

SKU:
RBDL00062
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O19052
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PPAR-G,PPARG1,PPARG2,NR1C3,Glitazone Receptor,Nuclear Receptor Subfamily 1 Group C Member 3
Reactivity:
Rabbit
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rabbit Peroxisome Proliferator Activated Receptor Gamma (PPARg) ELISA Kit

The Rabbit Peroxisome Proliferator-Activated Receptor Gamma (PPARG) ELISA Kit is specifically designed for the accurate measurement of PPARG levels in rabbit serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring trustworthy and consistent results for a variety of research purposes.PPARG is a key nuclear receptor involved in the regulation of various metabolic pathways, including lipid and glucose metabolism.

Dysregulation of PPARG has been linked to conditions such as obesity, diabetes, and cardiovascular diseases, making it a valuable biomarker for the study of these disorders and the development of potential treatments.With the Rabbit Peroxisome Proliferator-Activated Receptor Gamma (PPARG) ELISA Kit, researchers can confidently analyze PPARG levels in rabbit samples, aiding in the advancement of understanding and addressing metabolic diseases.

Product Name:Rabbit Peroxisome Proliferator Activated Receptor Gamma (PPARg) ELISA Kit
Product Code:RBDL00062
Size:96 Assays
Target:PPARg
Synonyms:PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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