**** The Rabbit MMP-2 (Matrix Metalloproteinase 2) ELISA Kit is a specialized assay meticulously designed for the quantitative detection of Matrix Metalloproteinase 2 (MMP-2) levels in various biological samples from rabbits.
MMP-2 is a critical enzyme involved in extracellular matrix remodeling and tissue homeostasis, making it a key player in processes like wound healing, angiogenesis, and tissue repair in rabbit models. Accurate measurement of MMP-2 levels is essential for understanding its role in physiological and pathological conditions, particularly in tissue remodeling and repair mechanisms in rabbits. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for assessing MMP-2 concentrations. Manufactured under high-quality control standards, this kit provides robust performance and user-friendly protocols, making it an excellent choice for research studies focused on matrix metalloproteinases and tissue biology in rabbits.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit MMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit MMP-2. You can calculate the concentration of Rabbit MMP-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
95-111
88-104
Average (%)
101
102
95
1:4
Range (%)
94-108
98-111
89-103
Average (%)
101
105
96
1:8
Range (%)
98-110
93-107
88-102
Average (%)
103
101
93
1:16
Range (%)
92-107
93-107
90-101
Average (%)
99
101
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.91
1.76
8.24
0.92
1.71
8.61
Standard deviation
0.05
0.08
0.33
0.05
0.08
0.46
C V (%)
5.49
4.55
4.0
5.43
4.68
5.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit MMP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit MMP-2 in samples. No significant cross-reactivity or interference between Rabbit MMP-2 and analogues was observed.
