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Rabbit Matrix Metalloproteinase 3 (MMP3) ELISA Kit

SKU:
RBDL00050
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P28863
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SL1,STMY,STMY1,STR1,Progelatinase,Stromelysin 1,Transin-1
Reactivity:
Rabbit
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rabbit Matrix Metalloproteinase 3 (MMP3) ELISA Kit

The Rabbit Matrix Metalloproteinase-3 (MMP3) ELISA Kit is a comprehensive tool designed for the precise measurement of MMP3 levels in rabbit serum, plasma, and tissue lysates. This kit offers unparalleled sensitivity and specificity, delivering consistent and accurate results for a variety of research needs.MMP3 is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a critical role in tissue remodeling and cell migration.

Aberrant MMP3 activity has been linked to various pathological conditions, including arthritis, cancer, and cardiovascular diseases, making it a valuable target for therapeutic interventions.With its robust performance and wide range of applications, the Rabbit MMP3 ELISA Kit is an essential resource for studying MMP3 biology and exploring its potential as a diagnostic or therapeutic target in various disease settings.

Product Name:Rabbit Matrix Metalloproteinase 3 (MMP3) ELISA Kit
Product Code:RBDL00050
Size:96 Assays
Target:MMP3
Synonyms:SL1, STMY, STMY1, STR1, Progelatinase, Stromelysin 1, Transin-1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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