The Rabbit LH (Luteinizing Hormone) ELISA Kit is a specialized assay designed to quantitatively detect levels of luteinizing hormone in various rabbit biological samples.
Luteinizing hormone is a critical hormone that plays a key role in the regulation of reproductive functions, such as the release of eggs in females and the production of testosterone in males. Accurate measurement of LH levels is essential for understanding reproductive health, fertility, and hormone regulation. This ELISA kit provides exceptional sensitivity and specificity, ensuring precise and reproducible results in the quantitative analysis of luteinizing hormone. Manufactured under rigorous quality control standards, the kit offers robust performance and ease of use, making it a valuable tool for researchers investigating reproductive endocrinology and hormone-related disorders.
Product Name:
Rabbit LH (Luteinizing Hormone) ELISA Kit
SKU:
AEES00575
Target:
Rabbit LH (Luteinizing Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 mIU/mL
Detection range:
1.56-100 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit LH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit LH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit LH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit LH. You can calculate the concentration of Rabbit LH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
95-110
90-101
Average (%)
92
102
95
1:4
Range (%)
88-103
81-93
83-97
Average (%)
95
85
90
1:8
Range (%)
91-102
82-92
87-101
Average (%)
96
87
92
1:16
Range (%)
87-101
82-94
85-98
Average (%)
93
89
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
99
EDTA plasma (n=5)
93-106
101
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.63
15.48
46.73
4.34
15.78
45.75
Standard deviation
0.29
0.83
2.26
0.27
0.91
1.84
C V (%)
6.26
5.36
4.84
6.22
5.77
4.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit LH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit LH in samples. No significant cross-reactivity or interference between Rabbit LH and analogues was observed.
