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Rabbit Insulin Like Growth Factor 1 (IGF1) ELISA Kit

SKU:
RBDL00029
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q95222
Sensitivity:
0.096ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
IGFI,IGF1A,IBP1,MGF,Somatomedin C,Mechano Growth Gactor
Reactivity:
Rabbit
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rabbit Insulin Like Growth Factor 1 (IGF1) ELISA Kit

The Rabbit Insulin-Like Growth Factor 1 (IGF1) ELISA Kit is specifically designed for the accurate and sensitive detection of IGF1 levels in rabbit serum, plasma, and tissue homogenates. This kit offers unparalleled sensitivity and specificity, allowing for precise and reproducible results in various research applications.IGF1 is a key growth factor that plays a crucial role in promoting cell growth, proliferation, and differentiation. It is involved in various physiological processes, including muscle growth, bone development, and tissue repair.

Dysregulation of IGF1 has been linked to numerous health conditions, such as diabetes, cancer, and growth disorders, making it a valuable biomarker for research and therapeutic development.With its advanced technology and reliable performance, the Rabbit IGF1 ELISA Kit is an indispensable tool for studying the role of IGF1 in different biological processes and diseases, providing valuable insights for potential diagnostic and therapeutic interventions.

Product Name:Rabbit Insulin Like Growth Factor 1 (IGF1) ELISA Kit
Product Code:RBDL00029
Size:96 Assays
Target:IGF1
Synonyms:IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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