**** The Rabbit INS (Insulin) ELISA Kit is a specialized assay meticulously crafted for the quantitative determination of insulin levels in a variety of rabbit biological samples. Insulin, a critical hormone produced by the pancreas, is paramount in regulating metabolism, glucose uptake, and energy homeostasis, making precise measurement of insulin levels essential for evaluating carbohydrate metabolism and overall health status in rabbits. Accurate quantification of insulin concentrations facilitates enhanced comprehension of glucose regulation, insulin resistance, and diabetic conditions in rabbits.
This ELISA kit delivers exceptional sensitivity and specificity, ensuring accurate and reproducible results for insulin quantitation. Manufactified under stringent quality control standards, the kit guarantees robust performance, making it an indispensable tool for research and diagnostic applications focusing on rabbit insulin levels.
Product Name:
Rabbit INS (Insulin) ELISA Kit
SKU:
AEES00587
Target:
Rabbit INS (Insulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit INS. You can calculate the concentration of Rabbit INS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
90-104
90-105
Average (%)
99
96
97
1:4
Range (%)
93-108
91-103
88-103
Average (%)
100
97
94
1:8
Range (%)
92-105
92-104
92-104
Average (%)
99
97
98
1:16
Range (%)
88-100
88-104
91-103
Average (%)
94
95
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.75
21.83
87.62
10.17
22.22
82.47
Standard deviation
0.65
1.13
2.92
0.61
0.93
4.35
C V (%)
6.67
5.18
3.33
6.0
4.19
5.28
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit INS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit INS in samples. No significant cross-reactivity or interference between Rabbit INS and analogues was observed.
