The Rabbit IL-6 ELISA Kit is a cutting-edge diagnostic tool designed for the precise measurement of interleukin-6 (IL-6) levels in diverse rabbit biological samples. IL-6 is a vital pro-inflammatory cytokine that partakes in a broad spectrum of cellular functions, including cell growth, differentiation, and immune responses.
Its pivotal role in mediating inflammatory reactions signifies its significance in the body's immune defense mechanisms and its implications in various inflammatory conditions, autoimmune diseases, and infections. Accurate quantification of IL-6 levels is fundamental in comprehending the intricate involvement of this cytokine in disease pathogenesis and in the development of targeted therapeutic approaches. The Rabbit IL-6 ELISA Kit enables researchers to obtain dependable and reproducible results, facilitating in-depth investigations into the functions and effects of IL-6 in rabbit health and disease. Engineered with exceptional sensitivity and specificity, this ELISA kit from Assay Genie ensures the accuracy and consistency of results. Manufactured under stringent quality control measures, this kit delivers robust performance and streamlined protocols, making it an ideal solution for research applications aimed at unraveling the role of IL-6 in various physiological and pathological processes in rabbits.
Product Name:
Rabbit IL-6 ELISA Kit
SKU:
AEES00565
Target:
Rabbit IL-6
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IL-6. You can calculate the concentration of Rabbit IL-6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
88-102
84-96
Average (%)
90
95
91
1:4
Range (%)
93-108
83-94
81-96
Average (%)
99
89
87
1:8
Range (%)
92-106
84-96
89-99
Average (%)
98
90
94
1:16
Range (%)
92-104
81-90
89-101
Average (%)
97
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-99
94
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.77
138.48
425.75
41.72
130.84
385.78
Standard deviation
2.6
7.92
18.73
2.78
6.15
19.29
C V (%)
5.68
5.72
4.4
6.66
4.7
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit IL-6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit IL-6 in samples. No significant cross-reactivity or interference between Rabbit IL-6 and analogues was observed.
