**** The Rabbit IL-4 ELISA Kit is a specialized assay designed for the quantitative measurement of Interleukin-4 (IL-4) levels in various biological samples from rabbits. IL-4 is a crucial cytokine that plays a key role in regulating immune responses, particularly the differentiation of T helper cells and B cells in rabbits. Accurate detection and quantification of IL-4 are essential for elucidating its immunomodulatory functions and understanding its impact on immune-related processes in rabbit models.
This ELISA kit provides exceptional sensitivity and specificity, ensuring precise and reproducible results in assessing IL-4 concentrations. Manufactured under strict quality control standards, this kit offers robust performance and ease of use, making it an excellent choice for research studies focused on the immune system and inflammatory responses in rabbits.
Product Name:
Rabbit IL-4 ELISA Kit
SKU:
AEES00582
Target:
Rabbit IL-4
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IL-4. You can calculate the concentration of Rabbit IL-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
98-110
86-102
Average (%)
103
104
93
1:4
Range (%)
95-112
100-114
91-105
Average (%)
102
106
96
1:8
Range (%)
96-110
100-112
86-96
Average (%)
101
106
91
1:16
Range (%)
99-112
95-113
86-100
Average (%)
105
103
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
91-103
96
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.66
133.82
463.08
57.47
124.06
458.41
Standard deviation
3.25
6.34
23.8
3.94
5.97
24.98
C V (%)
6.06
4.74
5.14
6.86
4.81
5.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit IL-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit IL-4 in samples. No significant cross-reactivity or interference between Rabbit IL-4 and analogues was observed.
