The Rabbit IL-1β ELISA Kit is an advanced assay that enables the precise quantification of interleukin-1 beta (IL-1β) levels in a variety of biological samples from rabbits.
IL-1β is a crucial pro-inflammatory cytokine that modulates various cellular functions, including cell growth, differentiation, and apoptosis. Its role in orchestrating inflammatory responses and regulating immune reactions makes it a key component in understanding the body's immune defense mechanisms and its involvement in a wide range of inflammatory conditions, autoimmune diseases, and infections. Accurate measurement of IL-1β levels provides researchers with essential information to delve deeper into the molecular mechanisms of inflammation-related diseases and design targeted therapeutic interventions. By utilizing the Rabbit IL-1β ELISA Kit, scientists can obtain reliable and reproducible data on IL-1β concentrations, facilitating comprehensive investigations into the cytokine's impact on health and disease. Crafted with exceptional sensitivity and specificity, this ELISA kit from Assay Genie delivers dependable and precise results. Manufactured under rigorous quality control standards, this kit offers robust performance and user-friendly procedures, making it an indispensable tool for research applications aimed at elucidating the intricate role of IL-1β in various physiological and pathological processes in rabbits.
Product Name:
Rabbit IL-1β ELISA Kit
SKU:
AEES00564
Target:
Rabbit IL-1β
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IL-1β. You can calculate the concentration of Rabbit IL-1β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
95-107
91-102
Average (%)
103
102
97
1:4
Range (%)
86-102
87-100
84-98
Average (%)
93
92
91
1:8
Range (%)
88-102
85-97
83-95
Average (%)
94
90
88
1:16
Range (%)
95-109
80-91
86-100
Average (%)
100
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
94-104
99
Cell culture media (n=5)
91-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.59
121.44
462.88
47.73
124.59
497.69
Standard deviation
2.65
7.16
14.86
3.08
6.28
23.64
C V (%)
5.57
5.9
3.21
6.45
5.04
4.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit IL-1β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit IL-1β in samples. No significant cross-reactivity or interference between Rabbit IL-1β and analogues was observed.
