Rabbit IgG (Immunoglobulin G) ELISA Kit (RBFI00157)

• Microplate reader with 450 nm wavelength filter
• Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
• Incubator
• Deionized or distilled water
• Absorbent paper
• Buffer resevoir

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Immunoglobulin G (IgG) is a vital component of the immune system, belonging to a class of antibodies known as gamma globulins. These antibodies are soluble proteins present in the bloodstream and other bodily fluids, playing a crucial role in defending against harmful pathogens.

The immunoglobulin heavy and light chains are coded for by three separate gene families. Each family is located on a separate chromosome - one for the heavy chain and one for each of the light chain types. Each of these gene families has several V region genes and one or more C region genes. The V and C regions genes are not however immediately adjacent to each other. This gene undergoes a process called somatic hypermutation, which generates a diverse array of antibodies with different binding affinities and specificities.

The structure of IgG is composed of four protein chains. These chains include two identical heavy chains and two identical light chains, linked together by disulfide bonds. Each chain comprises variable (V) and constant (C) regions. The V regions contribute to the antigen-binding site, enabling IgG to recognize and attach to specific foreign substances called antigens. The molecular weight of the rabbit IgG is 150kDa for their most common isotype.

The primary function of IgG is to provide protection against bacterial and viral infections. Plasma cells, derived from activated B cells, are responsible for producing IgG antibodies. These antibodies circulate within the bloodstream and lymphatic system, where they neutralize toxins, enhance the process of phagocytosis (engulfing and destroying pathogens), and facilitate the activation of other immune cells.

IgG possesses significant clinical significance. It is the most abundant antibody class in the human body and plays a crucial role in long-term immune memory. The presence or absence of IgG can offer valuable diagnostic insights into an individual's immune response. Elevated levels of IgG may indicate chronic infections, autoimmune diseases, or specific types of cancer. Conversely, reduced levels of IgG can be associated with primary immunodeficiencies or immunosuppression.

gG tests, also known as IgG antibody tests, are commonly employed to assess an individual's immune response to specific antigens. These tests measure the quantity of IgG antibodies present in the blood, helping determine if a person has been exposed to a particular pathogen or allergen. IgG testing is widely used in diagnosing infections, monitoring vaccine effectiveness, and identifying allergies, including food allergies.

The IgG index, also known as the IgG synthesis rate, is a calculation used in medicine to assess the presence of increased immunoglobulin G (IgG) levels within the central nervous system (CNS). It is primarily used as a diagnostic tool in the evaluation of neurological disorders, particularly those suspected to be associated with inflammation or an immune response.The IgG index is calculated by comparing the concentration of IgG in the cerebrospinal fluid (CSF) to the concentration of IgG in the blood.

An elevated IgG index suggests increased production of IgG within the CNS, indicating a possible immune response or inflammation. This finding can be useful in diagnosing conditions such as multiple sclerosis (MS), neurosyphilis, or other infectious or inflammatory disorders affecting the CNS. However, it is important to note that an elevated IgG index alone is not diagnostic of any specific condition and should be interpreted in conjunction with other clinical findings and diagnostic tests.

While IgE antibodies are widely recognized as the primary antibodies involved in immediate allergic reactions, some alternative practitioners and testing laboratories claim that elevated levels of IgG antibodies to specific foods can indicate food intolerances or sensitivities. However, scientific evidence supporting the use of IgG Food sensitivity tests for diagnosing food allergies is limited and inconclusive. Major allergy organizations and medical societies generally do not endorse or recommend IgG testing as a standalone diagnostic tool for food allergies. They argue that the presence of IgG antibodies to a specific food does not necessarily indicate an allergic reaction or intolerance, as IgG antibodies can be present even in individuals without symptoms or clinical significance.