The Rabbit IgE (Immunoglobulin E) ELISA Kit is specifically designed for the quantitative detection of IgE levels in various rabbit biological samples. Immunoglobulin E, known as IgE, is an essential antibody that is involved in allergic responses and plays a pivotal role in the immune system's defense against parasitic infections and hypersensitivity reactions. Measurement of IgE levels in biological samples is crucial for understanding allergic responses, diagnosing allergies, and monitoring treatment outcomes.
This ELISA kit offers exceptional sensitivity and specificity, allowing for accurate and reproducible results in the detection and quantification of IgE. Manufactured under rigorous quality control standards, this kit ensures robust performance and ease of use, making it an excellent choice for researchers interested in allergy-related studies and immunological research.
Product Name:
Rabbit IgE (Immunoglobulin E) ELISA Kit
SKU:
AEES00573
Target:
Rabbit IgE (Immunoglobulin E)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit IgE. You can calculate the concentration of Rabbit IgE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
95-107
97-110
Average (%)
106
101
103
1:4
Range (%)
89-103
86-99
85-97
Average (%)
94
92
90
1:8
Range (%)
91-107
85-97
82-94
Average (%)
98
91
88
1:16
Range (%)
88-104
84-98
87-99
Average (%)
95
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-107
101
EDTA plasma (n=5)
91-105
99
Cell culture media (n=5)
94-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.52
8.38
39.56
5.63
8.74
37.2
Standard deviation
0.37
0.43
1.28
0.36
0.5
1.46
C V (%)
6.7
5.13
3.24
6.39
5.72
3.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit IgE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit IgE in samples. No significant cross-reactivity or interference between Rabbit IgE and analogues was observed.
