**** The Rabbit hs-CRP (high-sensitivity C-Reactive Protein) ELISA Kit is a cutting-edge diagnostic tool engineered for the precise quantification of high-sensitivity C-reactive protein (hs-CRP) levels in diverse rabbit biological samples.
High-sensitivity CRP is a key biomarker of inflammation and a marker of systemic inflammation in rabbits, making it an indispensable parameter for assessing inflammatory and cardiovascular conditions with heightened precision. Accurate measurement of hs-CRP concentrations aids in elucidating the inflammatory status and evaluating the risk factors associated with various diseases and conditions in rabbits, including cardiovascular disorders and infections. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible outcomes for quantitative hs-CRP analysis. Manufactured under strict quality control measures, the kit guarantees robust performance and user-friendly operation, making it an optimal choice for research studies and diagnostic applications targeting hs-CRP levels in rabbits.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit hs-CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit hs-CRP. You can calculate the concentration of Rabbit hs-CRP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
91-104
87-98
Average (%)
98
97
93
1:4
Range (%)
88-103
90-105
88-103
Average (%)
94
97
94
1:8
Range (%)
93-110
94-105
90-102
Average (%)
100
99
96
1:16
Range (%)
94-106
91-101
88-100
Average (%)
99
96
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
83-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.25
315.28
736.5
93.63
340.62
781.77
Standard deviation
5.94
18.38
31.82
5.13
18.26
29.71
C V (%)
6.3
5.83
4.32
5.48
5.36
3.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit hs-CRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit hs-CRP in samples. No significant cross-reactivity or interference between Rabbit hs-CRP and analogues was observed.
