The Rabbit D-Dimer D2D ELISA Kit from Assay Genie is a powerful tool for detecting and measuring levels of D-Dimer in rabbit samples. With high sensitivity and specificity, this kit provides accurate and reliable results for research applications related to coagulation and thrombosis.D-Dimer is a fibrin degradation product that serves as a marker for blood clot formation and breakdown. Elevated levels of D-Dimer are associated with various conditions such as deep vein thrombosis, pulmonary embolism, and disseminated intravascular coagulation.
By accurately measuring D-Dimer levels in rabbit samples, researchers can gain valuable insights into the development and progression of these disorders.Whether studying coagulation disorders, investigating thrombotic events, or developing new therapies, the Rabbit D-Dimer D2D ELISA Kit offers a convenient and efficient solution for accurate D-Dimer detection in rabbit samples. Trust Assay Genie for reliable results in your research endeavors.
Product Name:
Rabbit D-Dimer (D2D) ELISA Kit
Product Code:
RBDL00021
Size:
96 Assays
Target:
D2D
Synonyms:
D 2 Dimer
Detection Method:
Competition
Range:
3.125-200ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-102
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
