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Rabbit Creatine Kinase, Muscle (CKM) ELISA Kit

SKU:
RBDL00016
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P00563
Sensitivity:
9.7U/L
Range:
15.625-1000U/L
ELISA Type:
Sandwich
Synonyms:
CK-MM,CKMM,CK3,CK-M,MM-CK,M-CK,Creatine kinase M-type, N-terminally processed
Reactivity:
Rabbit
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rabbit Creatine Kinase, Muscle (CKM) ELISA Kit

The Rabbit Creatine Kinase Muscle (CKM) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of CKM levels in rabbit serum, plasma, and tissue samples. CKM is an important enzyme found in muscle tissue, playing a key role in energy metabolism and muscle function.This ELISA kit is ideal for researchers studying muscle-related diseases, exercise physiology, and muscle injury.

With its reliable and reproducible results, this kit provides valuable insights into CKM levels, allowing for a better understanding of muscle health and function in rabbits.Whether investigating muscle disorders, monitoring athletic performance, or studying muscle metabolism, the Rabbit CKM ELISA Kit offers a powerful tool for researchers seeking to explore the role of CKM in muscle physiology.

Product Name:Rabbit Creatine Kinase, Muscle (CKM) ELISA Kit
Product Code:RBDL00016
Size:96 Assays
Target:CKM
Synonyms:CK-MM, CKMM, CK3, CK-M, MM-CK, M-CK, Creatine kinase M-type, N-terminally processed
Detection Method:Sandwich
Range:15.6-1,000U/L
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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