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Rabbit C3a DIY ELISA Kit

SKU:
KES0136
Product Type:
ELISA Kit
Reactivity:
Rabbit
Applications:
ELISA
ELISA Type:
DIY ELISA
Size:
10 Plates
Synonyms:
C3, AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 / C3a
€1,259
Frequently bought together:

Description

system_update_altDatasheet

Rabbit C3a DIY ELISA Kit

Based on the information in the URL provided, the Rabbit C3a ELISA Kit is an essential tool for detecting and quantifying levels of C3a in rabbit serum, plasma, and cell culture supernatants. This kit is specifically designed for researchers looking to study the role of C3a in inflammation, immune responses, and various disease conditions.C3a is a key component of the complement system, playing a critical role in inflammation and immune responses. Elevated levels of C3a have been associated with various inflammatory conditions, making it a valuable biomarker for studying and monitoring these diseases.

The Rabbit C3a ELISA Kit offered by Assay Genie boasts high sensitivity and specificity, ensuring accurate and reliable results for your research needs. With its user-friendly protocol and robust performance, this kit is ideal for a wide range of experimental applications and can provide valuable insights into the role of C3a in disease pathology and therapeutic interventions.

Product Name:Rabbit C3a DIY ELISA
Product Code:KES0136
Species:Rabbit
Target:C3a
Synonyms:C3, AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 / C3a
Application:ELISA
ELISA Type:DIY ELISA Kit
Size:10 Plates
Shipping:Room temperature
Storage:Stable for up to twelve months from date of receipt at 2-8°C.
Description Usage Quantity
Anti-Rabbit C3a Polyclonal Antibody Capture Antibody 100 µg
Biotinylated Anti-Rabbit C3a Polyclonal Antibody Detection Antibody 50 µg
Rabbit C3a Recombinant Protein Standard 5 µg
Reagent Suggested Formulation
DPBS: 0.008M sodium phosphate, 0.002M potassium phosphate, 0.14M sodium chloride, 0.01M potassium chloride, pH 7.4
96-well ELISA Plate: Clear, flat-bottom, high-binding 96-well plate, 8-wells per strip, 350 µL per well
(ELISA Plates: KESAP003)
Standard and Sample Diluent: The optimal Standard and Sample Diluent will need to be determined for each sample type to obtain optimal recovery and linearity. The appropriate Standard and Sample Diluent will mimic the sample's response to a known quantity of protein standard and will provide linear results when diluted. Often a 1:4 dilution of the sample in Reagent Diluent will provide acceptable recovery and linearity.
Reagent Diluent and Blocking Buffer: 4% BSA in DPBS, 0.2 µm filtered
Wash Buffer: 0.05% Tween®-20 in DPBS
Streptavidin-HRP: Enzymatic reagent to react with biotinylated detection antibody
(Streptavidin-HRP: KESAP002)
Substrate: 3,3',5,5'-tetramethylbenzidine (TMB) Substrate
(ELISA Accessory Pack: KESAP001)
Stop Solution: 0.18 M Sulfuric Acid
(ELISA Accessory Pack: KESAP001)
Plate Sealer: Adhesive film to prevent evaporation

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step Procedure
1. Prepare Capture Antibody in DPBS at desired working concentration.
2. Add 100 µL of Capture Antibody Working Solution to appropriate wells.
3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 12-24 hours.
4. Empty Capture Antibody Working Solution from plate. Blot plate onto paper towels or other absorbent material.
5. Add 100 µL of Blocking Buffer to appropriate wells.
6. Cover plate with Plate Sealer and incubate at room temperature for 1-3 hours.
7. Empty Blocking Buffer from plate. Blot plate onto paper towels or other absorbent material.
8. Prepare Standard and sample as desired with Standard and Sample Diluent.
9. Add 100 µL of Standard or sample to appropriate wells.
Note: Run each Standard or sample in duplicate.
10. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
11. Wash plate FOUR times with Wash Buffer.
Note: Gently squeeze the long sides of plate frame before washing to ensure all strips remain securely in the frame. Empty plate contents. Use a squirt wash bottle to vigorously fill each well completely with 1X Wash Buffer, then empty plate contents. Repeat procedure three additional times for a total of FOUR washes. Blot plate onto paper towels or other absorbent material.
12. Prepare Detection Antibody in Reagent Diluent at desired working concentration.
13. Add 100 µL of Detection Antibody Working Solution to each well.
14. Cover plate with Plate Sealer and incubate at room temperature for 1 hour.
15. Wash plate FOUR times with Wash Buffer as described in step 11.
16. Prepare Streptavidin-HRP in Reagent Diluent at desired working concentration.
17. Add 100 µL of Streptavidin-HRP Working Solution to each well.
18. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
19. Wash plate FOUR times with Wash Buffer as described in step 11.
20. Add 100 µL of TMB Substrate Solution to each well.
21. Develop the plate in the dark at room temperature for 30 minutes or as desired.
Note: Do NOT cover plate with Plate Sealer.
22. Stop reaction by adding 100 µL of Stop Solution to each well.
23. Measure absorbance on a plate reader at 450 nm.

The Rabbit C3a DIY ELISA kit from Assay Genie can assay for C3a in the following samples: serum, blood, plasma, cell culture supernatant and other related supernatants and tissues. Rabbit C3a DIY ELISA Kit from Assay Genie allows researchers to develop their own ELISA plates for C3a using our unique combination of capture and detection antibodies.

Each Rabbit C3a DIY ELISA Kit contains capture antibody, standard, and detection antibody for development of an C3a ELISA. C3a antibodies in this kit have been determined to function in an ELISA with the C3a standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined and require optimisation for the development of this kit. A working knowledge of ELISA is strongly recommended.

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