Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit
The Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit is a valuable tool for the precise measurement of BMP4 levels in rabbit serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.BMP4 is a key signaling molecule that plays a crucial role in the regulation of bone and cartilage development, as well as in embryonic development and tissue repair processes. Dysregulation of BMP4 expression has been linked to various pathological conditions, making it a significant biomarker for studying skeletal disorders, organ development, and tissue regeneration.
By utilizing the Rabbit BMP4 ELISA Kit, researchers can gain valuable insights into the role of BMP4 in health and disease, leading to a better understanding of its potential therapeutic applications. This kit is user-friendly, cost-effective, and designed to deliver reliable data, making it an essential tool for any laboratory conducting BMP4-related research.
Product Name:
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit
Product Code:
RBDL00012
Size:
96 Assays
Target:
BMP4
Synonyms:
BMP2B, BMP2-B, BMP2B1, ZYME, DVR4, Bone morphogenetic protein 2B
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
