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Rabbit Atrial Natriuretic Peptide (ANP) ELISA Kit

SKU:
RBDL00006
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07500
Sensitivity:
6.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
ANH,ANF,CDD,Atrial Natriuretic Factor,Atrial Natriuretic Hormone,Atriopeptin,Cardionatrine,Cardiodilatin
Reactivity:
Rabbit
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rabbit Atrial Natriuretic Peptide (ANP) ELISA Kit

The Rabbit Atrial Natriuretic Peptide (ANP) ELISA Kit is a powerful tool for accurately measuring ANP levels in rabbit samples such as serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit provides researchers with reliable and reproducible results for a wide range of experimental needs.ANP is a vital hormone that plays a key role in regulating blood pressure, fluid balance, and electrolyte homeostasis. Dysregulation of ANP levels has been linked to various cardiovascular diseases, making it a valuable biomarker for studying heart health and developing new treatments.

Whether you are conducting basic research or clinical studies, the Rabbit ANP ELISA Kit offers a user-friendly and efficient solution for accurately quantifying ANP levels in rabbit samples. Trust Assay Genie to provide you with the tools you need for successful cardiovascular research.

Product Name:Rabbit Atrial Natriuretic Peptide (ANP) ELISA Kit
Product Code:RBDL00006
Size:96 Assays
Target:ANP
Synonyms:ANH, ANF, CDD, Atrial Natriuretic Factor, Atrial Natriuretic Hormone, Atriopeptin, Cardionatrine, Cardiodilatin
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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