The V5 Tag Polyclonal Antibody (CABE071) is a high-quality antibody designed for research involving the V5 tag, a commonly used epitope tag for protein detection and purification. The antibody, raised in rabbits, exhibits high specificity and sensitivity in detecting V5-tagged proteins in various experimental applications, such as Western blotting and immunofluorescence.The V5 tag is a short, versatile tag that allows for the detection and purification of recombinant proteins, making it a valuable tool in protein expression and localization studies. The V5 Tag Polyclonal Antibody enables researchers to easily visualize and quantify V5-tagged proteins in cell lysates and tissue samples, providing valuable insights into protein expression levels and subcellular localization.
With its high affinity and specificity for the V5 tag, this antibody is an essential tool for researchers studying protein expression, localization, and protein-protein interactions in a wide range of biological systems. Its reliable performance and consistent results make it an indispensable reagent for any laboratory conducting protein research.
Product Name:
Rabbit anti V5-Tag Polyclonal Antibody
SKU:
CABE071
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
A synthetic peptide corresponding to V5 tag.
Sequence:
GKPI PNPL LGLD ST
Tested Applications:
WB
Recommended Dilution:
WB,1:1000 - 1:2000
Synonyms:
V5;V5 tag;V5-tag
Positive Sample:
293T
Observed MW:
Refer to Figures
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with V5-Wnt3a3d protein,using Rabbit anti V5-Tag pAb (AE071) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
