Rabbit anti V5-Tag Polyclonal Antibody (CABE071)
- SKU:
- CABE071
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Description
| Product Name: | Rabbit anti V5-Tag Polyclonal Antibody |
| SKU: | CABE071 |
| Size: | 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Species independent |
| Immunogen: | A synthetic peptide corresponding to V5 tag. |
| Sequence: | GKPIPNPLLGLDST |
| Tested Applications: | WB |
| Recommended Dilution: | WB,1:1000 - 1:2000 |
| Synonyms: | V5;V5 tag;V5-tag |
| Positive Sample: | 293T |
| Observed MW: | Refer to Figures |
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of normal 293T cells and 293T transfected with V5-Wnt3a3d protein,using Rabbit anti V5-Tag pAb (AE071) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min. |
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