The Rabbit Anti-Myc Tag Polyclonal Antibody (CABE009) is a valuable tool for researchers studying proteins tagged with the Myc epitope, a commonly used tag in molecular biology research. This antibody, raised in rabbits, has high reactivity with the Myc tag and is validated for use in various applications, including Western blotting and immunoprecipitation.The Myc tag is a short amino acid sequence that can be fused to a target protein to facilitate detection and purification. The Rabbit Anti-Myc Tag Polyclonal Antibody specifically binds to the Myc tag, allowing for easy detection and analysis of tagged proteins in a variety of cell types and experimental conditions.
By using the Rabbit Anti-Myc Tag Polyclonal Antibody, researchers can reliably study the expression, localization, and interactions of Myc-tagged proteins, advancing our understanding of cellular processes and protein function. Whether investigating protein-protein interactions, protein trafficking, or gene expression, this antibody is a valuable tool for researchers in molecular biology, cell biology, and biochemistry.
Product Name:
Rabbit anti Myc-Tag Polyclonal Antibody
SKU:
CABE009
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
A synthetic peptide corresponding to Myc tag.
Sequence:
EQKL ISEE DL
Tested Applications:
WBIF/ICCIP
Recommended Dilution:
WB,1:2000 - 1:6000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
Myc;Myc tag;Myc-tag
Positive Sample:
293T-NLK-Myc
Conjugate:
Unconjugated
Observed MW:
60kDa
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein,using Rabbit anti Myc-Tag pAb (AE009) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
