Rabbit anti His-Tag Polyclonal Antibody (CABE068)
- SKU:
- CABE068
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Description
| Product Name: | Rabbit anti His-Tag Polyclonal Antibody |
| SKU: | CABE068 |
| Size: | 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Species independent |
| Immunogen: | A synthetic peptide corresponding to His tag. |
| Sequence: | HHHHHH |
| Tested Applications: | WB IF/ICC IP |
| Recommended Dilution: | WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
| Synonyms: | His;His tag;His-tag |
| Positive Sample: | 293T |
| Observed MW: | 35kDa |
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (AE068) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s. |
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