The HA Tag Polyclonal Antibody (CABE036) is a high-quality antibody designed for research involving the HA tag, a small epitope that is widely used in protein research for labeling and detection. This antibody, raised in rabbits, is highly specific and sensitive, making it ideal for a variety of applications including immunoprecipitation, immunofluorescence, and flow cytometry.The HA tag is commonly used to facilitate the identification and purification of recombinant proteins in cell biology and molecular biology research.
The HA Tag Polyclonal Antibody recognizes the HA tag sequence with high affinity, enabling researchers to easily detect and study proteins tagged with this epitope in various experimental systems.With its versatility and reliability, the HA Tag Polyclonal Antibody is a valuable tool for researchers working in protein expression, protein purification, and protein localization studies. Its superior performance and specificity make it a trusted reagent for a wide range of applications in the field of molecular biology.
Product Name:
Rabbit anti HA-Tag Polyclonal Antibody
SKU:
CABE036
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
A synthetic peptide corresponding to HA-Tag.
Sequence:
YPVD VPDY A
Tested Applications:
WBIF/ICC
Recommended Dilution:
WB,1:2000 - 1:6000 IF,1:50 - 1:200
Synonyms:
HA;HA tag;HA-tag
Positive Sample:
293T
Conjugate:
Unconjugated
Observed MW:
26kDa
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Rabbit anti HA-Tag pAb (AE036) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
