The Rabbit Anti-GST Tag Polyclonal Antibody (CABE006) is a high-quality antibody designed for research involving the Glutathione S-Transferase (GST) tag, a commonly used fusion tag in protein expression and purification. This polyclonal antibody, raised in rabbits, is highly specific and sensitive for detecting GST-tagged proteins in various experimental applications such as Western blotting and immunoprecipitation.The GST tag is frequently used in recombinant protein research for easy purification and detection of target proteins. This antibody specifically recognizes the GST tag, allowing for efficient detection and analysis of GST-tagged proteins in cell lysates, protein extracts, and other experimental samples.
Its reliability and sensitivity make it a valuable tool for protein research, protein-protein interaction studies, and protein expression analysis.Researchers in the fields of molecular biology, biochemistry, and protein engineering will find this Rabbit Anti-GST Tag Polyclonal Antibody (CABE006) to be a valuable reagent for their studies. With its high specificity and sensitivity, this antibody provides accurate and dependable results, making it an essential tool for protein research and analysis.
Product Name:
Rabbit anti GST-Tag Polyclonal Antibody
SKU:
CABE006
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
Recombinant protein containing a sequence corresponding to amino acids 1-218 of GST protein.
WB,1:1000 - 1:2000 IP,3μg antibody for 50μg extracts of recombinant protein
Synonyms:
GST;GST tag;GST-tag
Positive Sample:
GST,GST fusion protein
Conjugate:
Unconjugated
Calculated MW:
26kDa
Observed MW:
27kDa
Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins. Members of the GST superfamily are extremely diverse in amino acid sequence, and a large fraction of the sequences deposited in public databases are of unknown function. The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.A GST-tag is often used to separate and purify proteins that contain the GST-fusion protein. The tag is 220 amino acids (roughly 26 KDa) in size, which, compared to tags such as the Myc-tag or the FLAG-tag, is quite large. It can be fused to either the N-terminus or C-terminus of a protein. However, many commercially available sources of GST-tagged plasmids include a thrombin domain for cleavage of the GST tag during protein purification.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of over-expressed GST protein using Rabbit anti GST-Tag pAb (AE006) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
