Rabbit anti DDDDK-Tag Polyclonal Antibody (CABE004)
The Rabbit Anti-DDDDK Tag Polyclonal Antibody (CABE004) is a valuable tool for researchers studying proteins tagged with the DDDDK epitope. This specific antibody, raised in rabbits, is highly reactive and specially designed for use in various applications such as Western blotting, immunofluorescence, and immunoprecipitation.The DDDDK tag, also known as the FLAG tag, is commonly used in protein research for easy detection and purification of recombinant proteins. The CABE004 antibody specifically recognizes and binds to the DDDDK tag, allowing for precise and sensitive detection of tagged proteins in cell lysates or tissue samples.
By using the Rabbit Anti-DDDDK Tag Polyclonal Antibody (CABE004), researchers can confidently study and analyze the expression, localization, and interaction of DDDDK-tagged proteins in a wide range of experimental settings. This antibody is a valuable tool for those working in molecular biology, cell biology, and protein biochemistry, providing reliable and reproducible results for their research projects.
Product Name:
Rabbit anti DDDDK-Tag Polyclonal Antibody
SKU:
CABE004
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Species independent
Immunogen:
A synthetic peptide corresponding to DDDDK tag.
Sequence:
DYKD DDDK
Tested Applications:
WBIF/ICCIP
Recommended Dilution:
WB,1:2000 - 1:10000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
DDDDK;DDDDK tag;DDDDK-tag
Positive Sample:
293T
Conjugate:
Unconjugated
Observed MW:
48kDa
FLAG-tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to obtain proteins of sufficient purity and quality to carry out 3D structure determination by x-ray crystallography.A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against a given protein, adding a FLAG-tag to a protein allows the protein to be studied with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis and Western blotting.
Purification Method:
Affinity purification
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with DDDDK-Tag using Rabbit anti DDDDK-Tag pAb(AE004) at 1:10000 dilution.Secondary antibody:(AS014 ) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 1s.
