The Rabbit Anti-Chicken IgY Fab (CABS088) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits strong reactivity with chicken IgY and is suitable for various applications such as ELISA, immunofluorescence, and immunohistochemistry.Chicken IgY is an important component of the avian immune system, serving as the primary antibody produced by chickens in response to antigens. By targeting IgY Fab fragments, researchers can gain insights into the immune response of chickens and study their antibody production in different experimental settings.
This antibody is validated for use in WB applications and has demonstrated reactivity against Chicken samples.
Product Name:
Rabbit Anti-Chicken IgY Fab
SKU:
CABS088
Size:
100μL
Reactivity:
Chicken
Clone Number:
ARC56601
Conjugate:
Unconjugated
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
WB
Recommended Dilution:
WB
,1:2000 - 1:20000
Positive Sample:
Chicken IgY Fab
Observed MW:
25kDa
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Chicken IgY Fab, using Rabbit Anti-Chicken IgY Fab (CABS088) at1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
