The RAB7 Antibody (CAB12784) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for use in Western blot applications.Rab7a is a small GTPase protein that plays a crucial role in the endocytic pathway, controlling the transport of proteins and lipids within the cell. Dysregulation of Rab7a has been implicated in various diseases, including neurodegenerative disorders and cancer. By targeting Rab7a with this polyclonal antibody, researchers can accurately detect and analyze its expression levels in different cell types, advancing our understanding of its function in health and disease.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
RAB7 Antibody
SKU:
CAB12784
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
RAB family members are small, RAS-related GTP-binding proteins that are important regulators of vesicular transport. Each RAB protein targets multiple proteins that act in exocytic / endocytic pathways. This gene encodes a RAB family member that regulates vesicle traffic in the late endosomes and also from late endosomes to lysosomes. This encoded protein is also involved in the cellular vacuolation of the VacA cytotoxin of Helicobacter pylori. Mutations at highly conserved amino acid residues in this gene have caused some forms of Charcot-Marie-Tooth (CMT) type 2 neuropathies.
Purification Method
Affinity purification
Gene ID
7879
RRID
AB_2759627
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RAB7 Rabbit pAb (CAB12784) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RAB7 Rabbit pAb (CAB12784 1:200). Western blot was performed from the immunoprecipitate using RAB7 Rabbit pAb (CAB12784) at a dilition of 1:1000.
