The Rab5B Polyclonal Antibody (CAB7447) is a vital tool for researchers studying Rab5B, a small GTPase protein involved in regulating endocytic pathways and membrane trafficking. This antibody, generated in rabbits, specifically recognizes Rab5B in human samples and has been validated for use in Western blot applications.Rab5B plays a crucial role in controlling vesicle fusion and trafficking within cells, making it essential for various cellular processes such as receptor internalization, protein sorting, and organelle dynamics. Dysregulation of Rab5B has been linked to diseases like cancer, neurodegenerative disorders, and infectious diseases, highlighting its importance in cellular homeostasis.
By using the Rab5B Polyclonal Antibody, researchers can study the localization, expression, and function of Rab5B in different cell types and conditions. This antibody enables the detection and analysis of Rab5B, providing valuable insights into its role in cell biology and disease pathology. It is a valuable tool for investigating the molecular mechanisms underlying cellular trafficking and identifying potential therapeutic targets for diseases associated with Rab5B dysfunction.
Product Name:
RAB5B Rabbit Polyclonal Antibody
SKU:
CAB7447
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human RAB5B (NP_002859.1).
Sequence:
YDIT NQET FARA KTWV KELQ RQAS PSIV IALA GNKA DLAN KRMV EYEE AQAY ADDN SLLF METS AKTA MNVN DLFL AIAK KLPK SEPQ NLGG AAGR SRGV D
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:
RAB5B
Positive Sample:
HeLa,SKOV3,Mouse ovary,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasmic side, Early endosome membrane, Lipid-anchor, Melanosome.
Calculated MW:
24kDa
Observed MW:
24kDa
Enables GDP binding activity; GTP-dependent protein binding activity; and GTPase activity. Involved in antigen processing and presentation and plasma membrane to endosome transport. Located in endosome and extracellular exosome.
Purification Method:
Affinity purification
Gene ID:
5869
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using RAB5B antibody (CAB7447) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
