The KO Validated Rab27a Polyclonal Antibody (CAB1934) is a powerful tool for researchers studying Rab27a, a key protein involved in regulating intracellular vesicle transport and secretion. This antibody, produced in rabbits, is highly specific and reacts with samples from various species, including human, mouse, and rat. Validated for use in a range of experimental techniques, including Western blotting and immunofluorescence, this antibody enables precise detection and analysis of Rab27a protein levels in different cell types and tissues. Its reliability and specificity make it an essential tool for studies in cell biology, neurobiology, and cancer research.
Rab27a is a crucial regulator of vesicle trafficking and secretion processes in cells, impacting various physiological functions such as neurotransmitter release, immune response modulation, and tumor cell invasion. Understanding the role of Rab27a in these processes is essential for uncovering potential therapeutic targets in diseases like neurological disorders, immune disorders, and cancer. The KO Validated Rab27a Polyclonal Antibody (CAB1934) provides researchers with a reliable tool to further investigate the mechanisms underlying Rab27a function and its implications in various pathologies.
Product Name:
[KO Validated] RAB27A Rabbit Polyclonal Antibody
SKU:
CAB1934
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human RAB27A (NP_899057.1).
Late endosome, Lipid-anchor, Lysosome, Melanosome, Membrane.
Calculated MW:
25kDa
Observed MW:
27kDa
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Purification Method:
Affinity purification
Gene ID:
5873
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and RAB27A knockdown (KD) U-87MG cells, using [KD Validated] RAB27A Rabbit pAb (CAB1934) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
