The R-Spondin-1 Polyclonal Antibody (CAB8289) is a valuable tool for researchers studying the R-Spondin-1 protein, a key regulator of the Wnt signaling pathway. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the R-Spondin-1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in developmental biology, stem cell research, and cancer biology.R-Spondin-1 is a secreted protein that functions as a potent activator of Wnt signaling, playing a critical role in organ development, tissue regeneration, and tumorigenesis.
Its ability to enhance Wnt signaling makes it a promising target for therapeutic interventions in diseases such as cancer, where dysregulation of Wnt signaling contributes to tumor growth and progression. By unraveling the mechanisms of R-Spondin-1 action, researchers can gain insights into its potential as a therapeutic target and develop novel strategies for treating Wnt-related disorders.
Product Name:
RSPO1 Rabbit Polyclonal Antibody
SKU:
CAB8289
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human RSPO1 (NP_001033722.1).
This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
284654
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from SKOV3 cells, using RSPO1 Rabbit pAb (CAB8289) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
