The QuickStep Rabbit PG (Progesterone) ELISA Kit is specifically designed for the precise quantification of progesterone levels in rabbit serum, plasma, and other biological samples. With its high sensitivity and specificity, this kit offers accurate and reliable results, making it an invaluable tool for researchers in various fields.Progesterone is a key hormone involved in the regulation of the female reproductive system, playing a crucial role in the menstrual cycle, pregnancy, and other physiological processes. Abnormal progesterone levels have been linked to infertility, pregnancy complications, and hormonal disorders, highlighting the importance of monitoring progesterone levels in research studies and clinical settings.
Whether investigating the reproductive health of rabbits or studying the impact of progesterone on different biological processes, the QuickStep Rabbit PG ELISA Kit provides researchers with a robust and efficient solution for progesterone detection. Trust in this kit to deliver accurate and consistent results, advancing your research towards valuable insights and potential therapeutic developments.
Product Name:
QuickStep Rabbit Progesterone ELISA Kit
Product Code:
QSES057
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rabbit
Sensitivity:
0.05 ng/mL
Range:
0.16-10 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Rabbit serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rabbit Pg are added to the micro ELISA plate wells. Rabbit Pg in samples (or standards) competes with a fixed amount of Rabbit Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Rabbit Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Rabbit Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
0.47
1.53
4.35
0.42
1.60
4.70
Standard deviation
0.03
0.06
0.19
0.02
0.07
0.23
CV (%)
5.35
4.10
4.39
5.45
4.18
4.88
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
89-104
95
EDTA plasma(n=8)
94-109
101
Samples were spiked with high concentrations of Rabbit Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
