The QuickStep Monkey PG Progesterone ELISA Kit is specifically designed for the precise measurement of progesterone levels in monkey serum, plasma, and tissue culture samples. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it the perfect tool for a variety of research applications.Progesterone is a key hormone involved in the regulation of the female reproductive system and plays a crucial role in fertility, pregnancy, and menstrual cycle regulation. Monitoring progesterone levels can provide valuable insights into reproductive health and can be essential in diagnosing and managing conditions such as infertility, polycystic ovary syndrome, and menopause.
Whether studying hormonal changes in monkeys, investigating reproductive disorders, or developing new therapies, the QuickStep Monkey PG Progesterone ELISA Kit offers a reliable solution for progesterone detection in monkey samples. Trust in this kit for accurate and detailed progesterone analysis in your research studies.
Product Name:
QuickStep Monkey Progesterone ELISA Kit
Product Code:
QSES042
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Monkey
Sensitivity:
14.37 pg/mL
Range:
31.25-2000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Progesterone
Application:
This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum and plasma.
Specificity:
This kit recognizes Pg in samples. No significant cross-reactivity or interference between Pg and analogues was observed.
Dilution Method:
It is recommended to dilute normal serum/plasma samples at 10 fold.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Monkey Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Monkey Pg are added to the micro ELISA plate wells. Monkey Pg in samples (or standards) competes with a fixed amount of Monkey Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Monkey Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Monkey Pg in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
109.74
195.09
867.52
117.81
195.76
783.35
Standard deviation
6.51
10.14
39.73
7.50
9.44
24.21
CV (%)
5.93
5.20
4.58
6.37
4.82
3.09
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
86-98
92
EDTA plasma(n=8)
89-101
96
Samples were spiked with high concentrations of Monkey Pg and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
