The QuickStep Human Postn (OSF-2, Periostin) ELISA Kit is a powerful tool for the precise quantification of Postn levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it perfect for a variety of research applications.Postn, also known as OSF-2 or Periostin, is a key protein involved in various biological processes, particularly in tissue development, wound healing, and cancer progression.
By measuring Postn levels, researchers can gain valuable insights into these processes and potentially uncover new therapeutic targets for a range of diseases.Invest in the QuickStep Human Postn (OSF-2, Periostin) ELISA Kit for reliable and high-quality data that will advance your research efforts in the fields of oncology, regenerative medicine, and beyond.
Product Name:
QuickStep Human Periostin (OSF-2) ELISA Kit
Product Code:
QSES027
Assay Type:
Sandwich
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Human
Sensitivity:
1.52 ng/mL
Range:
3.12-200 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Periostin
Application:
This ELISA kit applies to the in vitro quantitative determination of POSTN/OSF-2 concentrations in serum and plasma.
Specificity:
This kit recognizes POSTN/OSF-2 in samples. No significant cross-reactivity or interference between POSTN/OSF-2 and analogues was observed.
Dilution Method:
It is recommended to dilute normal serum/plasma samples at 20-100 fold.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POSTN/OSF-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human POSTN/OSF-2 are added to the micro ELISA plate wells. Human POSTN/OSF-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human POSTN/OSF-2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human POSTN/OSF-2. The concentration of Human POSTN/OSF-2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
1.99
6.27
13.47
2.17
6.59
13.68
Standard deviation
0.13
0.32
0.62
0.12
0.35
0.42
CV (%)
6.78
5.17
4.61
5.58
5.32
3.04
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
91-103
98
EDTA plasma(n=8)
84-99
91
Samples were spiked with high concentrations of Human POSTN/OSF-2 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
