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QuickStep Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit

SKU:
AEES00632
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Human
Sensitivity:
0.06 ng/mL
Range:
0.13-8 ng/mL
Sample Type:
Serum
$599
Frequently bought together:

Description

QuickStep Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit

The QuickStep Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit is a cutting-edge assay designed for the precise quantitative measurement of NGAL levels in human biological samples. NGAL, also known as lipocalin-2, is a critical protein involved in various biological processes, including immune response, inflammation, and kidney injury. As a promising biomarker, NGAL plays a crucial role in diagnosing acute kidney injury, renal dysfunction, and other conditions related to inflammation and injury.

This high-sensitivity ELISA kit enables researchers and clinicians to accurately measure NGAL levels, providing valuable insights into kidney function, early detection of renal injury, and monitoring disease progression. With exceptional sensitivity and specificity, this kit ensures reliable and reproducible results, offering a reliable tool for investigating NGAL expression in different physiological and pathological conditions. Manufactured under stringent quality control standards, the QuickStep Human NGAL ELISA Kit guarantees robust performance and ease of use, making it an ideal choice for studies focusing on kidney disorders, inflammation, and biomarker research. Trust in the accuracy and precision of this ELISA kit to advance your research in nephrology, inflammatory diseases, and diagnostics.

Product Name:QuickStep Human NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
Product Code:AEES00632
Assay Type:Sandwich
Format:96 Assays
Assay Time:2.5h
Reactivity:Human
Detection Range:0.13-8 ng/mL
Sensitivity:0.06 ng/mL
Sample Type & Sample Volume:Serum, plasma, urine, 50μL
Specificity:This kit recognizes Human NGAL in samples. No significant or interference between Human NGAL and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Human NGAL concentrations in serum, plasma, urine.Please consult technical support for the applicability if other biological fluids need to be tested.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGAL. Samples (or Standards) and biotinylated detection antibody specific for Human NGAL are added to the micro ELISA plate wells. Human NGAL would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGAL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human NGAL. You can calculate the concentration of Human NGAL in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable)8 wells x 12 strips2-8℃, 1 month
Reference Standard2 vialsDiscard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent1 vial, 20 mL2-8℃
Biotinylated Detection Ab Working Solution1 vial, 6 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Concentrated HRP Conjugate (100×)1 vial, 120 μL2-8℃ (Protect from light)
Substrate Reagent1 vial, 10 mL
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results

Neutrophil gelatinase associated lipocalin (NGAL) also known as Lipocalin 2 or Lcn2 is a 25 kDa, is a member of the lipocalin superfamily and a pleiotropic mediator of various inflammatory processes [1].NGAL, identified originally as a protein associated with matrix metalloproteinase 9 (MMP-9) of human neutrophils. Lipocalins are extracellular proteins which share a common tertiary structure that forms a barrel-like hydrophobic ligand binding site. When bound to MMP-9, NGAL protects it from proteolytic degradation sustaining the proteolytic activity of MMP-9. Neutrophil gelatinase-associated lipocalin (NGAL), is released by various cell types and is an attractive biomarker of inflammation, ischemia, infection, and kidney damage. Both intestinal and metabolic inflammation, as observed in obesity and related disorders, are associated with increased NGAL synthesis [2]. While NGAL in the intestinal tract regulates the composition of the gut microbiota and shows anti-inflammatory activities, it also exhibits proinflammatory activities in other experimental settings. Plasma NGAL is a novel marker of kidney function, which correlates to duration of end-stage renal failure (ESRD) and serum creatinine in uremic patients awaiting kidney transplantation. Plasma NGAL is associated with homocysteine in transplanted patients.

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