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QuickStep Human IgM (Immunoglobulin M) ELISA Kit

SKU:
AEES00638
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Human
Sensitivity:
1.02 ng/mL
Range:
7.80-500 ng/mL
Sample Type:
Serum
€499
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Description

QuickStep Human IgM (Immunoglobulin M) ELISA Kit

The QuickStep Human IgM (Immunoglobulin M) ELISA Kit is an advanced assay meticulously crafted for quantitative analysis of Immunoglobulin M levels in human biological samples. Immunoglobulin M (IgM) is a primary antibody involved in the early stages of the immune response, playing a crucial role in neutralizing pathogens and activating specific immune defenses. Monitoring IgM levels provides valuable insights into the body's immune function and can aid in the diagnosis and management of various infectious and autoimmune diseases.

With exceptional sensitivity and accuracy, this ELISA kit enables precise measurement of IgM levels, allowing researchers and clinicians to assess immune system activity and response to pathogens or antigens. IgM serves as a key indicator of the body's initial immune response, making its quantitative analysis essential for evaluating immune function, disease progression, and treatment efficacy. Manufactured under rigorous quality control measures, the QuickStep Human IgM ELISA Kit ensures reliable and reproducible results, providing researchers and healthcare professionals with a dependable tool for immune system evaluation. This user-friendly kit delivers robust performance and high specificity, making it a preferred choice for investigations in immunology, infectious diseases, and autoimmune conditions. Count on the accuracy and sensitivity of this ELISA kit to enhance your studies and clinical assessments of IgM-related immune responses.

Product Name:QuickStep Human IgM (Immunoglobulin M) ELISA Kit
Product Code:AEES00638
Assay Type:Sandwich
Format:96 Assays
Assay Time:2.5h
Reactivity:Human
Detection Range:7.80-500 ng/mL
Sensitivity:1.02 ng/mL
Sample Type & Sample Volume:Serum, plasma, urine, saliva, 50μL
Specificity:This kit recognizes Human IgM in samples. No significant or interference between Human IgM and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Human IgM concentrations in serum, plasma, urine, saliva.Please consult technical support for the applicability if other biological fluids need to be tested.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgM. Samples (or Standards) and biotinylated detection antibody specific for Human IgM are added to the micro ELISA plate wells. Human IgM would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IgM. You can calculate the concentration of Human IgM in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable)8 wells x 12 strips2-8℃, 1 month
Reference Standard2 vialsDiscard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent1 vial, 20 mL2-8℃
Biotinylated Detection Ab Working Solution1 vial, 6 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Concentrated HRP Conjugate (100×)1 vial, 120 μL2-8℃ (Protect from light)
Substrate Reagent1 vial, 10 mL
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results

Immunoglobulin M (IgM) is a class of immunoglobulins found in blood and lymph fluid that are the first type of antibody made in response to an infection [1]. IgM antibodies make up about 5 percent to 10 percent of all the antibodies in the body. IgM is principally concerned with the primary antibody response, appearing soon after initial invasion by an antigen and capable of destroying the antigen when it is first introduced. Its normal mean of serum concentration is 120 mg/dl. IgM is the first immunoglobulin expressed in the human fetus (around 20 weeks) and phylogenetically the earliest antibody to develop [2]. IgM antibodies appear early in the course of an infection and usually reappear, to a lesser extent, after further exposure. IgM antibodies do not pass across the human placenta (only isotype IgG). The biological properties of IgM make it useful in the diagnosis of infectious diseases. Demonstrating IgM antibodies in a patient's serum indicates recent infection, o

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