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QuickStep Human IgE (Immunoglobulin E) ELISA Kit

SKU:
AEES00641
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Human
Sensitivity:
0.27 ng/mL
Range:
0.78-50 ng/mL
Sample Type:
Serum and plasma
$599
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Description

QuickStep Human IgE (Immunoglobulin E) ELISA Kit

The QuickStep Human IgE (Immunoglobulin E) ELISA Kit is a cutting-edge assay designed to accurately quantify IgE levels in human biological samples. Immunoglobulin E is a critical antibody isotype known for its role in allergic responses and immune defense mechanisms. Elevated levels of IgE are associated with allergic conditions such as asthma, eczema, and allergic rhinitis, making it a vital biomarker in allergy diagnosis and monitoring.

This high-sensitivity ELISA kit provides researchers with a precise tool to measure IgE concentrations, enabling a deeper understanding of the immune response and allergic diseases. By quantifying IgE levels, scientists can investigate the dynamics of allergic reactions, assess sensitization profiles, and evaluate treatment outcomes. The quantification of IgE is essential in clinical research, allergy testing, and personalized medicine approaches aimed at managing allergic disorders. Manufactured under stringent quality control protocols, the QuickStep Human IgE ELISA Kit ensures accurate and reproducible results, making it a reliable instrument for scientific investigations. With exceptional sensitivity and specificity, this kit offers robust performance and ease of use, facilitating allergy research, diagnostic applications, and therapeutic developments in the field of immunology. Trust in the precision of this ELISA kit to advance your understanding of IgE-mediated immune responses and allergic conditions.

Product Name:QuickStep Human IgE (Immunoglobulin E) ELISA Kit
Product Code:AEES00641
Assay Type:Sandwich
Format:96 Assays
Assay Time:2.5h
Reactivity:Human
Detection Range:0.78-50 ng/mL
Sensitivity:0.27 ng/mL
Sample Type & Sample Volume:Serum and plasma, 50μL
Specificity:This kit recognizes Human IgE in samples. No significant or interference between Human IgE and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Human IgE concentrations in serum and plasma.Please consult technical support for the applicability if other biological fluids need to be tested.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgE. Samples (or Standards) and biotinylated detection antibody specific for Human IgE are added to the micro ELISA plate wells. Human IgE would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human IgE. You can calculate the concentration of Human IgE in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable)8 wells x 12 strips2-8℃, 1 month
Reference Standard2 vialsDiscard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent1 vial, 20 mL2-8℃
Biotinylated Detection Ab Working Solution1 vial, 6 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Concentrated HRP Conjugate (100×)1 vial, 120 μL2-8℃ (Protect from light)
Substrate Reagent1 vial, 10 mL
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results

Immunoglobulin E (IgE), named in 1968 [1], was the last of the five classes of human antibodies to be discovered, and today is commonly associated with the various manifestations of allergic disease. IgE’s receptor-binding activities present unique features. There are two principal receptors, FcεRⅠ, structurally homologous to other members of the FcγR family, and FcεRⅡ/CD23. Adult serum IgE levels range from 20 to 200IU/mL and are generally considered to be abnormally elevated when they are greater than 333IU/mL (800ng/mL). Common diseases associated with IgE elevation include allergic asthma, seasonal allergic rhinitis, atopic dermatitis, drug-induced interstitial pneumonia, bronchopulmonary aspergillosis, leprosy, pemphigoid and certain parasitic infections [2].

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