QuickStep Human CEA (Carcinoembryonic Antigen) ELISA Kit
The QuickStep Human CEA (Carcinoembryonic Antigen) ELISA Kit is a reliable and accurate tool for the detection of CEA levels in human serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity, providing robust and consistent results for a variety of research applications.CEA is a well-known biomarker for various types of cancer, particularly colorectal cancer, making it a valuable target for diagnostic and prognostic purposes.
The QuickStep Human CEA ELISA Kit allows researchers to study CEA levels in different biological samples, aiding in the understanding of cancer progression and response to treatments.With its user-friendly protocol and precise detection capabilities, the QuickStep Human CEA ELISA Kit is an essential tool for cancer research, offering accurate and reliable results for the study of CEA in oncology and beyond.
Product Name:
QuickStep Human CEA (Carcinoembryonic Antigen) ELISA Kit
Product Code:
QSES030
Assay Type:
Sandwich
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Human
Sensitivity:
0.47 ng/mL
Range:
0.78-50 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
CEA
Application:
This ELISA kit applies to the in vitro quantitative determination of CEA concentrations in serum and plasma.
Specificity:
This kit recognizes CEA in samples. No significant cross-reactivity or interference between CEA and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Human serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CEA. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human CEA are added to the micro ELISA plate wells. Human CEA in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human CEA. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human CEA. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
2.44
7.17
18.89
2.29
6.53
19.86
Standard deviation
0.13
0.40
0.60
0.13
0.26
0.66
CV (%)
5.16
5.51
3.16
5.79
4.01
3.32
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
93-107
100
EDTA plasma(n=8)
87-98
92
Samples were spiked with high concentrations of Human CEA and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
