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QuickStep Human ADP/Acrp30 (Adiponectin) ELISA Kit

SKU:
AEES00644
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Human
Sensitivity:
0.18 ng/mL
Range:
0.39-25 ng/mL
Sample Type:
Serum and plasma
€499
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Description

QuickStep Human ADP/Acrp30 (Adiponectin) ELISA Kit

The QuickStep Human ADP/Acrp30 (Adiponectin) ELISA Kit is a specialized assay designed to quantitatively measure Adiponectin levels in human biological samples. Adiponectin, also known as ADP or Acrp30, is a crucial adipokine that plays a key role in regulating various metabolic processes, including glucose regulation, lipid metabolism, and anti-inflammatory responses. This high-sensitivity ELISA kit is tailored to provide precise and accurate measurements of Adiponectin, offering researchers valuable insights into metabolic health, obesity-related conditions, and metabolic disorders. Adiponectin serves as a critical mediator in metabolic homeostasis and has been implicated in various diseases, such as diabetes, cardiovascular disease, and metabolic syndrome.

Manufactured with a focus on quality and performance, the QuickStep Human ADP/Acrp30 ELISA Kit delivers exceptional sensitivity and specificity, ensuring reproducible results for research studies and clinical investigations. With robust performance and user-friendly protocols, this kit is an excellent choice for researchers aiming to explore the intricate relationship between Adiponectin levels and metabolic health parameters. Trust this ELISA kit to provide reliable data for understanding the complexities of metabolic regulation and metabolic disorders influenced by Adiponectin levels.

Product Name:QuickStep Human ADP/Acrp30 (Adiponectin) ELISA Kit
Product Code:AEES00644
Assay Type:Sandwich
Format:96 Assays
Assay Time:2.5h
Reactivity:Human
Detection Range:0.39-25 ng/mL
Sensitivity:0.18 ng/mL
Sample Type & Sample Volume:Serum and plasma, 50μL
Specificity:This kit recognizes Human ADP/Acrp30 in samples. No significant or interference between Human ADP/Acrp30 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Human ADP/Acrp30 concentrations in serum and plasma.Please consult technical support for the applicability if other biological fluids need to be tested.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ADP/Acrp30. Samples (or Standards) and biotinylated detection antibody specific for Human ADP/Acrp30 are added to the micro ELISA plate wells. Human ADP/Acrp30 would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ADP/Acrp30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human ADP/Acrp30. You can calculate the concentration of Human ADP/Acrp30 in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable)8 wells x 12 strips2-8℃, 1 month
Reference Standard2 vialsDiscard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent1 vial, 20 mL2-8℃
Biotinylated Detection Ab Working Solution1 vial, 6 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Concentrated HRP Conjugate (100×)1 vial, 120 μL2-8℃ (Protect from light)
Substrate Reagent1 vial, 10 mL
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results

Adiponectin(Acrp30/ADP), is a hormone exclusively secreted by adipocytes that regulates energy homeostasis, and glucose and lipid metabolism[1]. In human, it is a 244 amino acid, 30kDa, protein encoded by gene apM1, that shares structural homology with collagen â…§ and â…© and complement factor C1q[2]. Adiponectin assembles into different complexes including trimers (low molecular weight), hexamers (middle molecular weight), and higher order oligomeric structures (high molecular weight) that may affect biological activity[3]. Adiponectin is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity. Research has shown that adiponectin is associated with many kinds of diseases, such as obesity, insulin resistance, type 2 diabetes, hyperlipidemia, hypertension, atherogenesis and renal disease[2].

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