The Chicken Testosterone ELISA Kit is specifically designed for the precise measurement of testosterone levels in chicken serum samples. This kit offers high sensitivity and specificity, providing researchers with reliable and consistent results for various research applications.Testosterone is a key hormone in chickens that plays a vital role in reproductive processes, muscle development, and overall growth. Monitoring testosterone levels in chicken samples can help researchers understand the impact of hormonal fluctuations on various physiological processes, making it a valuable tool for studies related to poultry health, growth, and reproduction.
With its easy-to-use format and reliable performance, the Chicken Testosterone ELISA Kit is an essential tool for researchers looking to investigate the hormonal regulation and physiological functions of testosterone in chickens.
Product Name:
QuickStep Chicken Testosterone ELISA Kit
Product Code:
QSES012
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Chicken
Sensitivity:
0.13 ng/mL
Range:
0.31-20 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Testosterone
Application:
This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum and plasma.
Specificity:
This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Chicken serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Chicken T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Chicken T are added to the micro ELISA plate wells. Chicken T in samples (or standards) competes with a fixed amount of Chicken T on the solid phase supporter for sites on the HRP linked detection antibody specific to Chicken T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Chicken T in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
1.08
2.81
7.71
1.03
2.92
7.17
Standard deviation
0.07
0.14
0.33
0.06
0.12
0.28
CV (%)
6.02
5.08
4.34
6.14
4.27
3.86
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
94-107
100
EDTA plasma(n=8)
92-105
99
Samples were spiked with high concentrations of Chicken T and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
