The QuickStep Chicken E2/Estradiol ELISA Kit is specifically designed for the quantitative detection of E2/Estradiol levels in chicken samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.E2/Estradiol is a vital hormone in chickens, playing a crucial role in reproductive functions, growth, and development. Monitoring E2/Estradiol levels can provide valuable insights into the hormonal regulation of poultry health and productivity.
This ELISA kit is essential for researchers studying avian endocrinology, reproductive biology, and poultry nutrition. Overall, the QuickStep Chicken E2/Estradiol ELISA Kit is a valuable tool for researchers seeking to explore the impact of E2/Estradiol on chicken physiology and to advance our understanding of avian reproductive health.
Product Name:
QuickStep Chicken Estradiol ELISA Kit
Product Code:
QSES010
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Chicken
Sensitivity:
3.18 pg/mL
Range:
7.81-500 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estradiol
Application:
This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum and plasma.
Specificity:
This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Chicken serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
24.21
79.58
188.18
26.48
84.77
193.74
Standard deviation
1.50
3.84
5.78
1.63
3.78
8.35
CV (%)
6.19
4.82
3.07
6.14
4.46
4.31
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
84-100
91
EDTA plasma(n=8)
89-103
97
Samples were spiked with high concentrations of Chicken E2 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
