The QuickStep Canine E2 (Estradiol) ELISA Kit is specially designed for the quantitative detection of estradiol levels in canine serum or plasma samples. This kit offers high sensitivity and specificity, providing accurate and reproducible results for researchers studying reproductive health in dogs.Estradiol, a type of estrogen, plays a vital role in the regulation of the estrous cycle, fertility, and overall reproductive health in female dogs. Abnormal estradiol levels can indicate underlying reproductive issues or diseases, making it a crucial biomarker for veterinarians and researchers working in the field of canine reproduction.
By using the QuickStep Canine E2 ELISA Kit, researchers can gain valuable insights into the hormonal balance and reproductive health of dogs, leading to a better understanding of canine reproduction and potential treatment options for reproductive disorders. With its user-friendly format and reliable performance, this kit is an essential tool for any study involving canine estradiol levels.
Product Name:
QuickStep Canine Estradiol ELISA Kit
Product Code:
QSES006
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Canine
Sensitivity:
6.37 pg/mL
Range:
15.63-1000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estradiol
Application:
This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum and plasma.
Specificity:
This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Canine serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
45.48
142.49
489.60
49.01
155.25
478.12
Standard deviation
2.62
6.28
15.08
3.32
7.27
22.14
CV (%)
5.77
4.41
3.08
6.78
4.68
4.63
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
90-103
96
EDTA plasma(n=8)
85-99
92
Samples were spiked with high concentrations of Canine E2 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
