The QuickStep Bovine E3 Estriol ELISA Kit is specifically designed for the precise measurement of E3 Estriol levels in bovine serum, plasma, and tissue culture samples. With its high sensitivity and specificity, this kit provides dependable and consistent results, making it a valuable tool for various research purposes.E3 Estriol is a key hormone in cattle related to reproductive health and fertility, making it essential for studying conditions such as estrus cycle monitoring, pregnancy diagnosis, and hormonal imbalance assessments.
By accurately quantifying E3 Estriol levels, researchers can gain insight into physiological changes and potential reproductive issues in bovines.Overall, the QuickStep Bovine E3 Estriol ELISA Kit is a reliable and efficient tool for researchers and veterinarians looking to explore and monitor the hormonal profiles of bovine subjects in various research and clinical settings.
Product Name:
QuickStep Bovine Estriol ELISA Kit
Product Code:
QSES004
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Bovine
Sensitivity:
1.11 pg/mL
Range:
3.13-200 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Estriol
Application:
This ELISA kit applies to the in vitro quantitative determination of E3 concentrations in serum and plasma.
Specificity:
This kit recognizes E3 in samples. No significant cross-reactivity or interference between E3 and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Bovine serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine E3 are added to the micro ELISA plate wells. Bovine E3 in samples (or standards) competes with a fixed amount of Bovine E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine E3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
9.45
23.51
97.50
9.29
24.35
103.48
Standard deviation
0.51
1.35
5.06
0.51
1.06
4.20
CV (%)
5.38
5.76
5.19
5.54
4.36
4.06
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
87-103
94
EDTA plasma(n=8)
89-104
95
Samples were spiked with high concentrations of Bovine E3 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
