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QuickStep Human CRP (C-Reactive Protein) ELISA Kit

SKU:
AEES00636
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Human
Sensitivity:
0.23 ng/mL
Range:
0.39-25 ng/mL
Sample Type:
Serum and plasma
€499
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Description

QuickStep Human CRP (C-Reactive Protein) ELISA Kit

The QuickStep Human CRP (C-Reactive Protein) ELISA Kit is a cutting-edge assay designed for the precise quantification of C-reactive protein levels in human biological samples. C-reactive protein (CRP) serves as a crucial biomarker for inflammation and is a key indicator of systemic inflammation. Elevated CRP levels have been associated with various inflammatory conditions, including infections, autoimmune diseases, and cardiovascular disorders, making it a valuable marker in clinical diagnostics and research. This high-sensitivity ELISA kit offers exceptional accuracy and reproducibility in measuring CRP concentrations, allowing researchers and healthcare professionals to assess inflammatory responses with confidence. By quantifying CRP levels, this kit provides valuable insights into the degree of inflammation, disease activity, and response to therapeutic interventions.

Manufactured under stringent quality control standards, the QuickStep Human CRP ELISA Kit delivers robust performance and reliable results, ensuring the precision required for clinical and research applications. With its user-friendly format and exceptional sensitivity, this kit is a top choice for investigating inflammatory processes, monitoring disease progression, and evaluating treatment outcomes. Trust in the accuracy and specificity of this ELISA kit to advance your research and clinical investigations in the realm of inflammation and related disorders.

Product Name:QuickStep Human CRP (C-Reactive Protein) ELISA Kit
Product Code:AEES00636
Assay Type:Sandwich
Format:96 Assays
Assay Time:2.5h
Reactivity:Human
Detection Range:0.39-25 ng/mL
Sensitivity:0.23 ng/mL
Sample Type & Sample Volume:Serum and plasma, 50μL
Specificity:This kit recognizes Human CRP in samples. No significant or interference between Human CRP and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum and plasma.Please consult technical support for the applicability if other biological fluids need to be tested.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) and biotinylated detection antibody specific for Human CRP are added to the micro ELISA plate wells. Human CRP would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable)8 wells x 12 strips2-8℃, 1 month
Reference Standard2 vialsDiscard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent1 vial, 20 mL2-8℃
Biotinylated Detection Ab Working Solution1 vial, 6 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Concentrated HRP Conjugate (100×)1 vial, 120 μL2-8℃ (Protect from light)
Substrate Reagent1 vial, 10 mL
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Product Description1 copy
Certificate of Analysis1 copy
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results

C-reactive protein (CRP) is a member of the phylogenetically ancient and highly conserved ‘pentraxin’ family of proteins, which also includes serum amyloid P component (SAP), a constituent of all amyloid deposits [1]. In man and other animal species, CRP is a major acute-phase plasma protein displaying rapid and pronounced rise of its serum concentration in response to infection or tissue injury.CRP is used mainly as a marker of inflammation. Apart from liver failure, there are few known factors that interfere with CRP production. Interferon alpha inhibits CRP production from liver cells which may explain the relatively low levels of CRP found during viral infections compared to bacterial infections [2]. Measuring and charting CRP values can prove useful in determining disease progress or the effectiveness of treatments. ELISA, immunoturbidimetry, nephelometry, rapid immunodiffusion, and visual agglutination are all methods used to measure CRP.

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