The QTRT1 Antibody (PAC042130) is a polyclonal antibody designed for research involving QTRT1, a protein involved in telomerase activity. This antibody, produced in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blotting and immunohistochemistry. It specifically binds to the QTRT1 protein, allowing for the detection and analysis of QTRT1 levels in different cell types.QTRT1 is a key component of the telomerase complex, which plays a crucial role in maintaining telomere length and ensuring genomic stability. Dysregulation of telomerase activity has been linked to aging, cancer, and other diseases, making QTRT1 a promising target for research and potential therapeutic interventions.
By studying the expression and function of QTRT1, researchers can gain valuable insights into telomerase biology and its implications in various physiological and pathological conditions. Overall, the QTRT1 Antibody (PAC042130) is an essential tool for researchers interested in exploring the role of QTRT1 in telomerase regulation, telomere maintenance, and related cellular processes. Its high specificity and sensitivity make it ideal for a wide range of research applications in the fields of molecular biology, cancer biology, and aging-related studies.
Western blot. All lanes: QTRT1 antibody at 6µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: PC-3 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 45, 25 kDa. Observed band size: 45 kDa.
Immunohistochemistry of paraffin-embedded human testis tissue using PACO42130 at dilution of 1:100.
Background:
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).
QTRT1: Interacts with QTRTD1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine). Belongs to the queuine tRNA-ribosyltransferase family.Protein type: Mitochondrial; RNA processing; EC 2.4.2.29; TransferaseChromosomal Location of Human Ortholog: 19p13.3Cellular Component: cytoplasm; mitochondrial outer membraneMolecular Function: queuine tRNA-ribosyltransferase activityBiological Process: queuosine biosynthetic process; tRNA modification
UniProt Protein Details:
NCBI Summary:
This gene encodes the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Feb 2012]
