Anti-QRSL1 Antibody (CAB8499)
- SKU:
- CAB8499
Frequently bought together:
Description
| Antibody Name: | Anti-QRSL1 Antibody |
| Antibody SKU: | CAB8499 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 289-528 of human QRSL1 (NP_060762.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 289-528 of human QRSL1 (NP_060762.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ELSS EVQS LWSK AADL FESE GAKV IEVS LPHT SYSI VCYH VLCT SEVA SNMA RFDG LQYG HRCD IDVS TEAM YAAT RREG FNDV VRGR ILSG NFFL LKEN YENY FVKA QKVR RLIA NDFV NAFN SGVD VLLT PTTL SEAV PYLE FIKE DNRT RSAQ DDIF TQAV NMAG LPAV SIPV ALSN QGLP IGLQ FIGR AFCD QQLL TVAK WFEK QVQF PVIQ LQEL MDDC SAVL ENEK LASV SLKQ |
| Gene ID: | 55278 |
| Uniprot: | Q9H0R6 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 32kDa/57kDa |
| Observed MW: | 57kDa |
| Synonyms: | QRSL1, GatA |
| Background: |
| UniProt Protein Function: | QRSL1: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu- tRNA(Gln). Belongs to the amidase family. GatA subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 6.3.5.7; Ligase Chromosomal Location of Human Ortholog: 6q21 Cellular Component: mitochondrion Molecular Function:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Biological Process: mitochondrial translation |
| UniProt Code: | Q9H0R6 |
| NCBI GenInfo Identifier: | 167016573 |
| NCBI Gene ID: | 55278 |
| NCBI Accession: | Q9H0R6.2 |
| UniProt Secondary Accession: | Q9H0R6,Q5VWJ4, Q9HA60, Q9NV19, |
| UniProt Related Accession: | Q9H0R6 |
| Molecular Weight: | 57kDa |
| NCBI Full Name: | Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial |
| NCBI Synonym Full Names: | glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 |
| NCBI Official Symbol: | QRSL1Â Â |
| NCBI Official Synonym Symbols: | GatAÂ Â |
| NCBI Protein Information: | glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial |
| UniProt Protein Name: | Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial |
| UniProt Synonym Protein Names: | Glutaminyl-tRNA synthase-like protein 1 |
| UniProt Gene Name: | QRSL1Â Â |
 | Western blot analysis of extracts of various cell lines, using QRSL1 antibody (CAB8499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |