The QPRT Antibody (CAB14349) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly sensitive to human samples and has been validated for use in Western blot applications. It binds specifically to the QPRT protein, allowing for the accurate detection and analysis of QPRT levels in various cell types.QPRT is a crucial enzyme involved in the production of nicotinamide adenine dinucleotide (NAD+), a molecule essential for cellular energy production and DNA repair.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
QPRT Antibody
SKU:
CAB14349
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
QPRTase, HEL-S-90n, QPRT
Positive Sample:
Mouse kidney, Rat liver
Cellular Localization:
Cytoplasm, Cytosol, Extracellular Exosome.
Calculated MW:
31kDa
Observed MW:
31kDa
This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
23475
RRID
AB_2761215
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using QPRT Rabbit pAb (CAB14349) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
