Pyruvate Kinase (PK) Activity Assay Kit (MAES0247)
- SKU:
- MAES0247
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Tissue And Cell
- Sensitivity:
- 0.08 U/L
- Range:
- 0.08-6.79 U/L
- Assay Time:
- 10 min
Description
| Product Name: | Pyruvate Kinase (PK) Activity Assay Kit |
| Product Code: | MAES0247 |
| Sample Type: | Serum, Plasma, Tissue And Cell |
| Assay Time: | 10 min |
| Assay Type: | Enzyme Activity |
| Synonyms: | PK |
| Detection Instrument: | Microplate reader (330-350 nm) |
| Sensitivity: | 0.08 U/L |
| Detection Range: | 0.08–6.79 U/L |
| Inter-assay CV: | 0.07 |
| Intra-assay CV: | 0.05 |
In the presence of adenosine diphosphate (ADP), pyruvate kinase (PK) catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, Lactate dehydrogenase catalyzes the reaction of pyruvic acid with NADH to produce lactic acid and NAD+. The activity of pyruvate kinase can be calculated by detection the absorbance changes at 340 nm.
| Storage: | -20°C |
| Valid Period: | 12 months |
| Other Instruments Required: | Test tube, Micropipettor, Vortex mixer, Incubator |
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