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Pyruvate Dehydrogenase (PDH) Activity Assay Kit

SKU:
MAES0243
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Urine, Animal Tissue, Cell
Sensitivity:
0.28 U /L
Range:
0.28-76.23 U/L
Assay Time:
15 min
€469
Frequently bought together:

Description

Product Name:Pyruvate Dehydrogenase (PDH) Activity Assay Kit
Product Code:MAES0243
Sample Type:Serum (Plasma), Urine, Animal Tissue, Cell
Assay Time:15 min
Assay Type:Enzyme Activity
Synonyms:PDH
Detection Instrument:Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Sensitivity:0.28 U /L
Detection Range:0.28-76.23 U/L
Inter-assay CV:0.1
Intra-assay CV:0.02

Pyruvate dehydrogenase (PDH) catalyzed the oxidative decarylation of pyruvate into acetyl CoA, which plays an important role in the metabolism of mitochondrial respiratory chain and is associated with the aging of cancer genes. Pyruvate can be decarboxylated by PDH under the action of CoA. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of PDH can be calculated by measuring the change of absorbance value at 450 nm.

Storage:-20°C
Valid Period:12 months
Other Instruments Required:Centrifuge, 37℃ incubator

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