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Pyruvate Decarboxylase (PDC) Activity Assay Kit

SKU:
MAES0260
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Serum, Plasma, Tissue And Cell Samples
Sensitivity:
0.67 U/L
Range:
0.67-27.73 U/L
Assay Time:
15 min
$587
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Description

Product Name:Pyruvate Decarboxylase (PDC) Activity Assay Kit
Product Code:MAES0260
Sample Type:Serum, Plasma, Tissue And Cell Samples
Assay Time:15 min
Assay Type:Enzyme Activity
Synonyms:PDC
Detection Instrument:Microplate reader (330-350 nm, optimum wavelength: 340 nm)
Detection Method:Colorimetric method
Sensitivity:0.67 U/L
Detection Range:0.67-27.73 U/L
Inter-assay CV:0.031
Intra-assay CV:0.028

Pyruvate decarboxylase (PDC) mainly exists in yeast and is one of the key enzymes in ethanol fermentation. PDC catalyzes pyruvate decarboxylate to acetaldehyde, which reacts under the action of ethanol dehydrogenase (ADH), and catalyzes NADH to convert into NAD+. NADH has a characteristic absorption peak at 340 nm. The PDC activity can be calculated by measuring the OD value at 340 nm.

Storage:-20°C
Valid Period:12 months
Other Reagents Required:Normal saline (0.9% NaCl)
Other Instruments Required:Vortex mixer, Centrifuge

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